Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

EXPERIMENTAL EYE RESEARCH | JUNE 2018

Volume 171, June 2018, Pages 164-173

Kate E Keller 1, Sanjoy K Bhattacharya 2, Theresa Borrás 3, Thomas M Brunner 4, Sunee Chansangpetch 5, Abbott F Clark 6, W Michael Dismuke 7, Yiqin Du 8, Michael H Elliott 9, C Ross Ethier 10, Jennifer A Faralli 11, Thomas F Freddo 12, Rudolf Fuchshofer 13, Michael Giovingo 14, Haiyan Gong 15, Pedro Gonzalez 16, Alex Huang 17, Murray A Johnstone 18, Paul L Kaufman 11, Mary J Kelley 1, Paul A Knepper 19, Casey C Kopczynski 7, John G Kuchtey 20, Rachel W Kuchtey 20, Markus H Kuehn 21, Raquel L Lieberman 10, Shan C Lin 5, Paloma Liton 16, Yutao Liu 22, Elke Lütjen-Drecoll 23, Weiming Mao 6, Marisse Masis-Solano 5, Fiona McDonnell 16, Colleen M McDowell 6, Darryl R Overby 24, Padmanabhan P Pattabiraman 25, Vijay K Raghunathan 26, P Vasanth Rao 16, Douglas J Rhee 25, Uttio Roy Chowdhury 27, Paul Russell 28, John R Samples 29, Donald Schwartz 30, Evan B Stubbs 31, Ernst R Tamm 13, James C Tan 32, Carol B Toris 25, Karen Y Torrejon 33, Janice A Vranka 1, Mary K Wirtz 1, Thomas Yorio 6, Jie Zhang 17, Gulab S Zode 6, Michael P Fautsch 34, Donna M Peters 35, Ted S Acott 36, W Daniel Stamer 37

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.